PurProX® AAV Purification Solution
Gene Therapy BrochureProduct Introduction & Advantages of PurProX® AAV Purification Solution
GeneMedi's PurProX® is GeneMedi’s proprietary, end-to-end modular system for AAV Downstream Processing (DSP).
Engineered to bridge the "Process Gap" between benchtop discovery and GMP manufacturing, PurProX® operates on a philosophy of "Unified Chemistry & Linear Scalability." The portfolio encompasses tools ranging from rapid benchtop preparation to kg-scale industrial production. Crucially, all capture products (Spin Columns, Pre-packed Columns, and Resins) are available in both U1 (Broad-Spectrum) and U2 (AAV9 and its variants) formats, delivering high-purity, high-recovery, and scalable vector solutions for every stage of gene therapy development.
PurProX® AAVPure Affinity Resins
Cat.No:
GMV-PurProX-AAVPure-U-1/2
GeneMedi PurProX® AAVPure Affinity Resin provide high-performance AAV purification.
U-1: resin broadly targets serotypes like AAV2/5/6/8/DJ/7m8 (with weaker binding for AAV9 compared with other AAV serotypes)
U-2: is specialized for high-affinity AAV9 and its variants binding.
PurProX® AAVPure Pre-packed Affinity Column
Cat.No:
GMV-PurProX-AAVPure-U-1/2-P
GeneMedi's PurProX® AAVPure Pre-packed Affinity Column provide a convenient, ready-to-use solution for researchers evaluating our AAV purification resin or performing small-scale AAV vector purification.
PurProX® AAVEasy rapid-to-purify Spin Column
Cat.No:
GMV-PurProX-AAVEasy-U-1/2-10T
Based on PurProX® AAVPure Affinity Resins, AAVEasy rapid-to-purify Spin Columns provide a rapid, equipment-free solution for small-scale AAV purification, delivering high-purity viral vectors in just 30 minutes.
PurProX® AAVFull Enrichment Kit
Cat.No:
GMV-PurProX-AAVFull-U-1
PurProX® AAVFull empowers you to effortlessly maximize full-capsid ratio with GeneMedi’s seamless Anion Exchange (AEX) chromatography Technology.
· Convenient Syringe Format
-Rapid, hassle-free handling using everyday lab tools.
· Targeted Clearance
-Specifically eliminates empty capsids to yield premium, ultra-pure AAV vectors.
· Ultracentrifuge-Free
-Achieve maximum full-capsid ratios right on your benchtop.
Product list for PurProX® AAV Purification Solution: Resins, Spin Columns, Pre-packed Columns & Full-Capsid Enrichment
| Product Type | Cat No | Product Name | Serotype | Size | Products Information |
|---|---|---|---|---|---|
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-1 | PurProX® AAVPure Affinity Resin-1 | Versatile resin for high-capacity, multi-serotype binding (e.g., AAV2/5/6/8/DJ/7m8) | 10 mL, 25 mL, 50 mL, 250 mL, 1 L, 5 L, 10 L | Details |
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-2 | PurProX® AAVPure Affinity Resin-2 | Optimized for AAV9 and variants like AAV-PHP series | 10 mL, 25 mL, 50 mL, 250 mL, 1 L, 5 L, 10 L | Details |
| Pre-packed Column | GMV-PurProX-AAVPure-U-1-P | PurProX® AAVPure Pre-packed Column-1 | Versatile resin for high-capacity, multi-serotype binding (e.g., AAV2/5/6/8/DJ/7m8) | 1mL,5mL | Details |
| Pre-packed Column | GMV-PurProX-AAVPure-U-2-P | PurProX® AAVPure Pre-packed Column-2 | Optimized for AAV9 and variants like AAV-PHP series | 1mL,5mL | Details |
| Spin Column (10 tests) | GMV-PurProX-AAVEasy-U-1-10T | PurProX® AAVEasy Spin Column-1 | Versatile resin for high-capacity, multi-serotype binding (e.g., AAV2/5/6/8/DJ/7m8) | 10T | Details |
| Spin Column (10 tests) | GMV-PurProX-AAVEasy-U-2-10T | PurProX® AAVEasy Spin Column-2 | Optimized for AAV9 and variants like AAV-PHP series | 10T | Details |
Validation Data using AAV Purification Platform
- PurProX® AAVPure Affinity Resin
- PurProX® AAVPure Pre-packed Column
- PurProX® AAVEasy Spin Columns
- PurProX® AAVFull Enrichment Filters
PurProX® AAVPure Affinity Resin
PurProX® AAVPure Affinity Resin-1 for AAV Serotype 2, 5, 6,8, DJ, and 7m8 Purification
The silver staining of purified AAVs by PurProX® AAVPure Affinity Resin-1 (GMV-PurProX-AAVPure-U-1). Company T‘s Resin is used as the control.
PurProX® AAVPure Affinity Resin-2 Designed for AAV9 Purification
The silver staining of purified AAV9 by PurProX® AAVPure Affinity Resin-2 (GMV-PurProX-AAVPure-U-2). Company T‘s Resin is used as the control.
PurProX® AAVPure Pre-packed Column
Fig1. AAV8 purified with AAV affinity resin by AKTA
Chromatogram illustrating the purification process of AAV8 serotype using the AAV affinity resin on the ÄKTA system. The x-axis represents time, while the y-axis shows absorbance (A280 for protein, A260 for nucleic acid) and conductivity. Peaks indicate sample loading, washing, and elution phases.
Fig2. Silver staining of AAV8 purification
Silver-stained SDS-PAGE gel showing protein bands from AAV8 purification fractions. Lanes include molecular weight markers, lysate, flow-through (FT), and elution fractions. Capsid proteins (VP1, VP2, VP3) are visible in the elution lane at ~60-80 kDa.
Fig3. Silver staining of AAV6 purification
Silver-stained SDS-PAGE gel depicting protein bands from AAV6 purification fractions. Lanes show markers, lysate, flow-through (FT), and elution, with AAV6 capsid proteins (VP1, VP2, VP3) prominent in the elution lane.
Table.1 Recovery of several serotypes of purified AAV
| Serotype | Sample | Vg | Recovery Rate (%) |
|---|---|---|---|
| AAV2 | Load | 5.72E+12 | / |
| Elution | 5.19E+12 | 90.73 | |
| AAV5 | Load | 3.36E+13 | / |
| Elution | 3.13E+13 | 93.15 | |
| AAV6 | Load | 8.28E+12 | / |
| Elution | 7.73E+12 | 93.36 | |
| AAV8 | Load | 1.33E+13 | / |
| Elution | 1.24E+13 | 93.67 |
PurProX® AAVEasy Spin Columns
Fig1. AAV5 Purification by PurProX® AAVEasy
Handling Time: 29 mins
Recovery Rate: 95.43%
PurProX® AAVEasy Spin Column-1 for AAV Serotype 2, 5, 6, 8, DJ, and 7m8 Purification
The silver staining of purified AAVs by PurProX® AAVEasy Spin Column-1 (GMV-PurProX-AAVEasy-U-1).
PurProX® AAVEasy Spin Column-2 Designed for AAV9 Purification
The silver staining of purified AAVs by PurProX® AAVEasy Spin Column-2 (GMV-PurProX-AAVEasy-U-2). Company T's Resin (AKTA purification) is used as the control.
PurProX® AAVFull Enrichment Filter
The AAV8 chromatogram demonstrates that this enrichment kit efficiently achieves separation of empty and full capsids, ensuring a final yield of highly pure AAV vectors with a high full-capsid ratio.
