PurProX™ Adeno-Associated Virus (AAV)
Purification Solutions
A one-stop high-performance platform from GeneMedi, encompassing affinity resins, prepacked columns, and rapid spin columns, providing high-purity, high-recovery solutions for gene therapy drug discovery and development.
Explore Product DetailsProduct Introduction and Advantages
GeneMedi'sPurProX™ AAV Purification Productsare high-performance solutions specifically designed for the isolation and purification of adeno-associated virus (AAV) vectors. We offer flexible solutions to meet various needs in early discovery, preclinical research, CMC process development, and clinical applications.
PurProX® AAVPure Affinity Resin
Cat.No: GMV-PurProX-AAVPure-U-1/2GeneMedi's PurProX™ AAVPure affinity resin provides high-performance AAV purification capabilities.
Two specificity options are available:
• U-1 resin:Broad-spectrum targetingAAV2, 5, 6, 8, DJ, 7m8and other serotypes (with weaker binding affinity to AAV9 compared to other serotypes).
• U-2 resin:Specifically designed forAAV9Designed for high-affinity binding.
PurProX® AAVPure Pre-packed AAV Affinity Purification Column
Cat.No: GMV-PurProX-AAVPure-U-1/2-PYinuo Biotechnology's 0.5mL PurProX™ AAVPure pre-packed affinity purification column provides researchers with a convenient, ready-to-use solution for evaluating our AAV purification resin performance or conducting small-scale AAV vector purification.
These purification columns are pre-packed with our high-performance PurProX™ AAVPure affinity resin, maintaining the same excellent binding capacity and serotype specificity as bulk resin, but in an optimized"plug-and-play"format.
Outstanding product performance
PurProX™ AAVPure Affinity Resin redefines the technical prowess of domestic products
Broad-spectrum affinity purification capability
Compatible with multiple AAV serotypes and various mutants, ensuring high flexibility in research and production.
High recovery rate
Recovery rate exceeds90%, minimizing sample waste and significantly increasing yield.
Reusable
Supports multiple regeneration cycles, significantly reducing production costs.
High Pressure Resistance and High Flow Rate
Pressure resistance exceeds5MPa, perfectly meeting the demands of industrial-scale production.
Batch-to-Batch Consistency
Delivers consistent quality with no ligand loss, ensuring reliability in every production outcome.
Cost Control Advantage
Performance rivals top global brands while offering significant cost-effectiveness, making it an ideal choice for the AAV gene therapy industry.
Rapid Purification Solution
PurProX® AAVEasy Rapid Purification Spin Column for AAV
Cat.No: GMV-PurProX-AAVEasy-U-1/2-10TBased onPurProX™ AAVPure Affinity ResinThe AAVEasy Rapid Purification Spin Column provides a fast, equipment-free solution for small-scale AAV purification, requiring only30 minutesto obtain high-purity viral vectors.
These disposable spin columns are designed for simplicity and consistency, eliminating the need for complex chromatography systems while maintaining extremely highrecovery rates (>90%)and purity.
The AAVEasy system features specific binding capabilities for multiple AAV serotypes. U-1 resin targetsAAV2, AAV5, AAV6, AAV8, AAV-DJ, and AAV-7m8, while U-2 resin is specifically optimized for high-affinity binding toAAV9. The system requires only a standard centrifuge, offering a simplified and efficient alternative to iodixanol ultracentrifugation for AAV purification.
- Quick and easy solution:Purification time <30 minutes, no HPLC/FPLC required.
- Single-use convenience:Ideal for small-scale AAV preparation, avoiding cross-contamination.
- Consistent recovery:Provides reliable yield for downstream applications.
- Ultracentrifugation-free operation:Achieves high-purity AAV preparation with only a standard benchtop centrifuge.
PurProX™ AAV Purification Product List
| Product Type | Catalog Number | Product Name | Applicable Serotype | Specification | Details |
|---|---|---|---|---|---|
| AAV Affinity Resin | GMV-PurProX-AAVPure-U-1 | PurProX® AAVPure Affinity Resin | AAV2, 5, 6, 8, DJ, 7m8 | 10 mL - 10 L | View |
| AAV affinity resin | GMV-PurProX-AAVPure-U-2 | PurProX® AAVPure affinity resin | AAV9 | 10 mL - 10 L | View |
| Pre-packed column | GMV-PurProX-AAVPure-U-1-P | PurProX® AAVPure Prepackaged Adeno-Associated Virus Affinity Purification Column | AAV2, 5, 6, 8, DJ, 7m8 | 1mL, 5mL | View |
| Prepackaged Column | GMV-PurProX-AAVPure-U-2-P | PurProX® AAVPure Prepackaged Adeno-Associated Virus Affinity Purification Column | AAV9 | 1mL, 5mL | View |
| Spin column | GMV-PurProX-AAVEasy-U-1-10T | PurProX® AAVEasy Adeno-Associated Virus Rapid Purification Spin Column | AAV2, 5, 6, 8, DJ, 7m8 | 10T | View |
| Spin column | GMV-PurProX-AAVEasy-U-2-10T | PurProX® AAVEasy Adeno-Associated Virus Rapid Purification Spin Column | AAV9 | 10T | View |
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Validation DataValidation Data
Figure 1. Purification of AAV8 using AAV affinity resin on the AKTA system
The chromatogram illustrates the purification process of AAV8 serotype using AAV affinity resin on the ÄKTA system. The X-axis represents time, while the Y-axis displays absorbance (A280 for protein, A260 for nucleic acid) and conductivity. The peaks clearly indicate the loading, washing, and elution phases.
Figure 2. Silver staining results of AAV8 purification
Silver-stained SDS-PAGE gel showing protein bands of AAV8 purification fractions. Lanes include molecular weight markers (Marker), lysate, flow-through (FT), and eluate. Capsid proteins (VP1, VP2, VP3) are clearly visible at ~60-80 kDa in the elution lane.
Figure 3. Silver staining results of AAV6 purification
Silver-stained SDS-PAGE gel showing protein bands of purified AAV6 components. Lanes display the marker, lysate, flow-through (FT), and eluate, with AAV6 capsid proteins (VP1, VP2, VP3) prominently visible in the elution lane.
Table 1. Recovery rates of several purified AAV serotypes
| Serotype | Sample | Genome copy number (Vg) | Recovery Rate (%) |
|---|---|---|---|
| AAV2 | Load | 5.72E+12 | / |
| Elution | 5.19E+12 | 90.73% | |
| AAV5 | Load | 3.36E+13 | / |
| Elution | 3.13E+13 | 93.15% | |
| AAV6 | Loading | 8.28E+12 | / |
| Elution | 7.73E+12 | 93.36% | |
| AAV8 | Loading | 1.33E+13 | / |
| Elution | 1.24E+13 | 93.67% |
