SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-free)
Order Information
| Catalog No. | Product Name | Size | Price (USD) |
|---|---|---|---|
| GM-CD8-T-Cell-iso-kit-CF-10T | SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-free) | 10 Tests | $190 |
| GM-CD8-T-Cell-iso-kit-CF-50T | SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-free) | 50 Tests | $570 |
| GM-CD8-T-Cell-iso-kit-CF-100T | SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-free) | 100 Tests | $1004 |
| GM-CD8-T-Cell-iso-kit-CF-750T | SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-free) | 750 Tests |
Specifications
- Cell Population CD8-T Cell
- Labeling Type Indirect
- Bead Type Non-releasable
- Format Column-free
- Isolation Method Negative CD8-T Cell Isolation (Untouched)
- Storage Store at 2℃ - 8℃
Description
| Catalog No. | GM-CD8-T-Cell-iso-kit-CF |
| Product Name | SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-free) |
| Description | SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-free) is a Indirect CD8+ T Cell Isolation Kit (Column-free, Non-releasable) |
| Application | SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-free) is used for Negative CD8+ T Cell Isolation (Untouched), with the Key Features, such as Gentle negative selection; maintains cell viability; suitable for sensitive downstream applications |
| Components |
|
| Labeling Type | Indirect |
| Bead Type | Non-releasable |
| Format | Column-free |
| Key Feature | Gentle negative selection; maintains cell viability; suitable for sensitive downstream applications |
| Cell Population | CD8-T Cell |
| Storage | Store at 2℃ - 8℃ |
Validation Data
Superior Isolation of High-Purity CD8+ T Cells from Human PBMCs Using SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-free)
Catalog No. GM-CD8-T-Cell-iso-kit-CF
To evaluate the isolation efficiency of the SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-free), CD8+ T cells were enriched from human peripheral blood mononuclear cells (hPBMCs) via negative selection. In this column-free protocol, non-target cells were labeled with a biotinylated antibody cocktail followed by magnetic beads. The labeled cells were then sequestered by a magnetic separator, allowing the highly purified, untouched CD8+ T cells to be recovered directly from the supernatant.
Cell fractions were stained with CD3-APC and CD8-PE for flow cytometric analysis. The frequency of CD8+ T cells demonstrated a substantial increase from 12.1% in the pre-isolation sample to 93.8% in the post-isolation fraction. These results confirm that the column-free system provides effective depletion of non-target populations, yielding high-purity CD8+ T cells while maintaining their native state and maximal viability.
Figure 19. Rapid isolation and high-purity enrichment of Human CD8+ T Cells Using SOLIDEX®-ISOEx Untouched Human CD8+ T Cell Isolation Kit (Column-free). Representative flow cytometry plots showing the frequency of CD8+ T cells in hPBMCs before isolation (12.1%) and after enrichment via column-free negative selection (93.8%).
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