SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based)
Achieve superior research results with the SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit. Designed for high-efficiency Human NK Cell purification from PBMC, this kit utilizes a specialized Column-based method with Magnetic Beads to ensure exceptional recovery and purity (up to 94.3%). Our Untouched (Negative) selection strategy leaves target NK cells completely antibody- and bead-free, preserving their native state for functional assays. Ideal for immunology and cytotoxicity studies requiring High Purity and zero activation.
SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) is a Indirect NK Cell Isolation Kit (Column-based, Non-releasable)
Order Information
| Catalog No. | Product Name | Size | Price (USD) |
|---|---|---|---|
| GM-NK-Cell-iso-kit-10T | SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) | 10 Tests | USD 190.00 |
| GM-NK-Cell-iso-kit-50T | SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) | 50 Tests | USD 570.00 |
| GM-NK-Cell-iso-kit-100T | SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) | 100 Tests | USD 1,004.00 |
| GM-NK-Cell-iso-kit-750T | SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) | 750 Tests |
Specifications
- Cell Population NK Cell
- Labeling Type Indirect
- Bead Type Non-releasable
- Format Column-based
- Isolation Method Negative NK Cell Isolation (Untouched)
- Storage Store at 2℃ - 8℃
Description
| Catalog No. | GM-NK-Cell-iso-kit |
| Product Name | SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) |
| Description | SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) is a Indirect NK Cell Isolation Kit (Column-based, Non-releasable) |
| Application | SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) is used for Negative NK Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies |
| Components |
|
| Labeling Type | Indirect |
| Bead Type | Non-releasable |
| Format | Column-based |
| Key Feature | Preserves native cell function; maintains surface marker integrity; ideal for functional studies |
| Cell Population | NK Cell |
| Storage | Store at 2℃ - 8℃ |
Application
SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-based) is used for Negative NK Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Protocol
- Preparation: Resuspend PBMCs in separation buffer.
- Labeling: Add the Isolation Cocktail and incubate for 15 minutes at 2-8°C.
- Washing: Wash cells with buffer to remove unbound antibodies.
- Bead Incubation: Add Anti-Biotin Nanobeads and incubate for 15 minutes at 2-8°C.
- Separation: Pass the suspension through the LS Column; collect the flow-through (enriched NK cells)
Validation Data
High-Purity Isolation of NK Cells from Human PBMCs Using SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based)
Catalog No.GM-Tg-hg-NK-Cell-iso-kit
Human peripheral blood mononuclear cells (PBMCs) were freshly recovered and subjected to NK cell isolation using SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based) and Company M’s NK Cell kit. The isolated cell population was analyzed by flow cytometry to evaluate purity and recovery rate. SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based) provides superior performance in isolating NK cells from human PBMCs, delivering high purity and recovery rate for downstream applications.
Figure 6. High-Purity Isolation of NK Cells from Human PBMCs Using SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-Based). The flow cytometry analysis of the isolated cells from cryopreserved PBMCs.
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Background
Why Choose SOLIDEX®-ISOEx?
- Untouched Purity: Delivers high purity (validated data shows increase from ~8% to >94%) while keeping NK cells free of labels.
- Preserved Functionality: The negative selection strategy ensures zero activation, maintaining the cell's native killing capacity.
- Column-Based Efficiency: The column amplifies the magnetic field, ensuring superior depletion of non-target cells compared to column-free methods.
- Fast Workflow: Optimized protocol with short incubation times allowing rapid isolation.
FAQ
Q1: What are the sample requirements? Can I use frozen PBMCs?
The kit is optimized for human PBMCs. Both fresh and cryopreserved PBMCs can be used. However, for frozen samples, ensure high viability (>90%) and remove clumps/debris with a strainer before labeling to prevent column clogging.
Q2: Since this is an Untouched selection, can I stain the cells immediately after isolation?
Yes. Because the NK cells in the flow-through are "untouched" (no antibodies or beads bound to them), all surface receptors are available. You can immediately proceed with flow cytometry staining or functional activation.
Q3: How does the Column-based separation work?
The column contains a matrix that amplifies the magnetic field. Magnetically labeled non-target cells are retained inside the column, while the unlabeled (untouched) NK cells pass through freely.
Q4: Can the columns be reused?
No. Columns are designed for single-use only to ensure sterility and prevent cross-contamination between samples.
Q5: What is the advantage of this Column-based method over Column-free methods?
Column-based isolation typically achieves higher purity because the column matrix physically traps weakly magnetic contaminants that might escape in a simple tube-based magnet system. It also allows for thorough washing to remove background noise.
Q6: Is this protocol compatible with automation?
Yes, the magnetic separation steps can be adapted to standard automated magnetic liquid handling workstations.
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