SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-free)
Order Information
| Catalog No. | Product Name | Size | Price (USD) |
|---|---|---|---|
| GM-NK-Cell-iso-kit-CF-10T | SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-free) | 10 Tests | $190 |
| GM-NK-Cell-iso-kit-CF-50T | SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-free) | 50 Tests | $570 |
| GM-NK-Cell-iso-kit-CF-100T | SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-free) | 100 Tests | $1004 |
| GM-NK-Cell-iso-kit-CF-750T | SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-free) | 750 Tests |
Specifications
- Cell Population NK Cell
- Labeling Type Indirect
- Bead Type Non-releasable
- Format Column-free
- Isolation Method Negative NK Cell Isolation (Untouched)
- Storage Store at 2℃ - 8℃
Description
| Catalog No. | GM-NK-Cell-iso-kit-CF |
| Product Name | SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-free) |
| Description | SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-free) is a Indirect NK Cell Isolation Kit (Column-free, Non-releasable) |
| Application | SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-free) is used for Negative NK Cell Isolation (Untouched), with the Key Features, such as Gentle negative selection; maintains cell viability; suitable for sensitive downstream applications |
| Components |
|
| Labeling Type | Indirect |
| Bead Type | Non-releasable |
| Format | Column-free |
| Key Feature | Gentle negative selection; maintains cell viability; suitable for sensitive downstream applications |
| Cell Population | NK Cell |
| Storage | Store at 2℃ - 8℃ |
Validation Data
Superior Isolation of High-Purity NK Cells from Human PBMCs Using SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-free)
Catalog No. GM-NK-Cell-iso-kit-CF
To evaluate the isolation efficiency of the SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-free), NK cells were enriched from human peripheral blood mononuclear cells (hPBMCs) via negative selection. In this column-free protocol, non-target cells were labeled with a biotinylated antibody cocktail followed by magnetic beads. The labeled cells were then sequestered by a magnetic separator, allowing the highly purified, untouched NK cells to be recovered directly from the supernatant.Cell fractions were stained with CD56-PE and CD3-APC for flow cytometric analysis. The frequency of NK cells demonstrated a substantial increase from 19% in the pre-isolation sample to 94.6% in the post-isolation fraction. These results confirm that the column-free system provides effective depletion of non-target populations, yielding high-purity NK cells while maintaining their native state and maximal viability for demanding downstream functional assays.
Figure 20. Rapid isolation and high-purity enrichment of Human NK Cells Using SOLIDEX®-ISOEx Untouched Human NK Cell Isolation Kit (Column-free).Representative flow cytometry plots showing the frequency of NK cells in hPBMCs before isolation (19%) and after enrichment via column-free negative selection (94.6%).
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