SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads (Column-based)
Unlock high-efficiency isolation of Human CD4+ T Cells (Helper T Cells) with SOLIDEX®-ISOEx Nanobeads. Utilizing a robust Positive Selection strategy, this kit specifically targets CD4 antigens to recover high-yield cell populations directly from PBMC. The Column-based format combined with biodegradable Magnetic Beads allows for rigorous washing, ensuring High Purity and minimal background. The gentle isolation process preserves cell viability, making it ideal for HIV research, cytokine analysis, and immune profiling.
SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads (Column-based) is a Direct CD4+ T Cell Isolation Nanobeads (Column-based, Non-releasable)
Order Information
| Catalog No. | Product Name | Size | Price (USD) |
|---|---|---|---|
| GM-Tg-hg-T10191-iso-nanoIMB-10T | SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads (Column-based) | 10 Tests | $112 |
| GM-Tg-hg-T10191-iso-nanoIMB-50T | SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads (Column-based) | 50 Tests | $336 |
| GM-Tg-hg-T10191-iso-nanoIMB-100T | SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads (Column-based) | 100 Tests | $591 |
| GM-Tg-hg-T10191-iso-nanoIMB-750T | SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads (Column-based) | 750 Tests |
Specifications
- Cell Population CD4-T Cell
- Labeling Type Direct
- Bead Type Non-releasable
- Format Column-based
- Isolation Method Positive CD4-T Cell Selection
- Storage Store at 2℃ - 8℃
Description
| Catalog No. | GM-Tg-hg-T10191-iso-nanoIMB |
| Product Name | SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads (Column-based) |
| Description | SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads (Column-based) is a Direct CD4+ T Cell Isolation Nanobeads (Column-based, Non-releasable) |
| Application | SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads (Column-based) is used for Positive CD4+ T Cell Selection, with the Key Features, such as Fast isolation; high purity; ideal for automated workflows |
| Labeling Type | Direct |
| Bead Type | Non-releasable |
| Format | Column-based |
| Key Feature | Fast isolation; high purity; ideal for automated workflows |
| Cell Population | CD4-T Cell |
| Storage | Store at 2℃ - 8℃ |
Application
SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads (Column-based) is used for Positive CD4+ T Cell Selection, with the Key Features, such as Fast isolation; high purity; ideal for automated workflows
Protocol
- Preparation: Prepare a single-cell suspension from Human PBMCs (fresh or frozen).
- Labeling: Add CD4 Microbeads directly to the cell suspension. Incubate to bind CD4+ T Cells.
- Separation: Place a separation column in the magnetic field and apply the cell suspension.
- Washing: Magnetically labeled CD4+ cells are retained in the column; unlabeled cells (CD8 T cells, B cells, etc.) are washed away.
- Elution: Remove the column from the magnet and elute the purified CD4+ T Cells.
Validation Data
Efficient Isolation of CD4+ T Cells Using SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads (Column-Based)
Catalog No. GM-Tg-hg-T10191-iso-nanoIMB
To assess the purity of isolated CD4+ T cells, magnetic separation was performed on human peripheral blood mononuclear cells (PBMCs). Both pre- and post-isolation cells were labeled with CD3-APC and CD4-PE antibodies and subjected to flow cytometric analysis. The purity of CD4+ T cells was significantly enhanced, rising from 28.1% before isolation to 97.0% after isolation, indicating highly efficient enrichment of CD4+ T cells.
Figure 9. High-Efficiency Enrichment of CD4+ Cells with SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads (Column-Based). Cells were incubated with anti-CD4 antibody-conjugated magnetic nanobeads and passed through an isolation column for positive selection. Flow cytometry shows CD4+ T cells in the input PBMC population and the resulting highly purified fraction.
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Background
Why Choose SOLIDEX®-ISOEx Human CD4 Isolation Nanobeads?
- Maximum Recovery: Positive selection efficiently captures target cells even from samples with low frequency.
- High Specificity: Column technology enables thorough washing, significantly reducing non-specific binding compared to clump-based methods.
- Bio-friendly Nanobeads: Biodegradable beads minimize receptor cross-linking, preventing artificial activation of sensitive Helper T cells.
- Standardized Workflow: Reliable results in under 45 minutes, compatible with downstream cell culture.
FAQ
What are the sample requirements?
The kit is optimized for fresh or cryopreserved Human PBMCs. It is also highly effective with Leukapheresis products (Leukopaks). Ensure cells are well-dispersed and viable (>90%) for best results.
Do the magnetic beads affect cell activation?
No. The SOLIDEX® nanobeads are biodegradable and nano-sized. They are engineered to bind without inducing significant signal transduction or activation, ensuring the CD4+ cells remain phenotypically unaltered for functional assays.
How does the Column-based separation work?
The column contains a matrix of ferromagnetic spheres. When placed in a magnetic field, these spheres amplify the magnetic gradient, trapping the bead-labeled CD4+ cells. This strong retention allows for vigorous washing of contaminants before the target cells are released.
Are the separation columns reusable?
No. To maintain sterility and absolutely prevent cross-contamination between donor samples, the columns are designed for single-use only.
What is the advantage of Column-based over Column-free methods?
Column-based positive selection generally delivers higher purity. The physical column matrix allows for the washing away of non-specific cells that might get trapped in the "clumps" formed by column-free tube methods.
Is this protocol compatible with automated systems?
Yes, the SOLIDEX®-ISOEx column format is designed to be compatible with most standard automated magnetic cell separators using column technology.
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