SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based)
Order Information
| Catalog No. | Product Name | Size | Price (USD) |
|---|---|---|---|
| GM-Tg-hg-T56365-dep-kit-10T | SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based) | 10 Tests | $112 |
| GM-Tg-hg-T56365-dep-kit-50T | SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based) | 50 Tests | $336 |
| GM-Tg-hg-T56365-dep-kit-100T | SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based) | 100 Tests | $591 |
| GM-Tg-hg-T56365-dep-kit-750T | SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based) | 750 Tests |
Specifications
- Cell Population B Cell
- Labeling Type Indirect
- Bead Type Non-releasable
- Format Column-based
- Isolation Method B Cell Depletion
- Storage Store at 2℃ - 8℃
Description
| Catalog No. | GM-Tg-hg-T56365-dep-kit |
| Product Name | SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based) |
| Description | SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based) is a Indirect B Cell Depletion Kit (Column-based, Non-releasable) |
| Application | SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based) is used for B Cell Depletion, with the Key Features, such as Specific cell depletion; minimal non-specific binding; optimal for complex samples |
| Components |
|
| Labeling Type | Indirect |
| Bead Type | Non-releasable |
| Format | Column-based |
| Key Feature | Specific cell depletion; minimal non-specific binding; optimal for complex samples |
| Cell Population | B Cell |
| Storage | Store at 2℃ - 8℃ |
Validation Data
Efficient Depletion of Human B Cells Using the SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based)
Catalog No. GM-Tg-hg-T56365-dep-kit
To evaluate the depletion efficiency of the SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based), human PBMCs were incubated and labeled with Biotin-CD19 antibodies and anti-biotin nanobeads, followed by magnetic column separation. The unlabeled target cells passed through the separation column and were collected, while the magnetically labeled CD19+ B cells were retained within the column. Following the magnetic separation, both pre- and post-depletion cell samples were analyzed by flow cytometry. The flow cytometry plots demonstrated that the initial proportion of B cells in the pre-depletion PBMC sample was 10.1%, whereas the residual B cell proportion in the flow-through fraction after efficient depletion plummeted to 0.091%. The logarithmic depletion for this process reached 2.14. This indicates that the product is capable of highly efficient depletion of non-target B cells.
Figure 23. Efficient depletion of human B cells using the SOLIDEX®-ISOEx Indirect Human CD19+ B Cell Depletion Kit (Column-based). In this experiment, CD19+ B cells were magnetically sorted and depleted from PBMCs. The results show a significant decrease in the proportion of CD19+ B cells in the sample, dropping from 10.1% pre-depletion to 0.091% after depletion.
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