SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads (Column-based)
Maximize your cell recovery with SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads. Employing a highly efficient Positive Selection strategy, this kit is designed to isolate Human CD3+ T Cells directly from PBMC with exceptional yield. The Column-based technology paired with specific Magnetic Beads allows for rigorous washing, resulting in High Purity cell populations. The biodegradable nanobeads ensure minimal interference, making the isolated cells ideal for molecular analysis and cell expansion.
SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads (Column-based) is a Direct Pan-T Cell Isolation Nanobeads (Column-based, Non-releasable)
Order Information
| Catalog No. | Product Name | Size | Price (USD) |
|---|---|---|---|
| GM-Tg-hg-T87075-iso-nanoIMB-10T | SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads (Column-based) | 10 Tests | $112 |
| GM-Tg-hg-T87075-iso-nanoIMB-50T | SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads (Column-based) | 50 Tests | $336 |
| GM-Tg-hg-T87075-iso-nanoIMB-100T | SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads (Column-based) | 100 Tests | $591 |
| GM-Tg-hg-T87075-iso-nanoIMB-750T | SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads (Column-based) | 750 Tests |
Specifications
- Cell Population Pan-T Cell
- Labeling Type Direct
- Bead Type Non-releasable
- Format Column-based
- Isolation Method Positive Pan-T Cell Selection
- Storage Store at 2℃ - 8℃
Description
| Catalog No. | GM-Tg-hg-T87075-iso-nanoIMB |
| Product Name | SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads (Column-based) |
| Description | SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads (Column-based) is a Direct Pan-T Cell Isolation Nanobeads (Column-based, Non-releasable) |
| Application | SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads (Column-based) is used for Positive Pan-T Cell Selection, with the Key Features, such as Fast isolation; high purity; ideal for automated workflows |
| Labeling Type | Direct |
| Bead Type | Non-releasable |
| Format | Column-based |
| Key Feature | Fast isolation; high purity; ideal for automated workflows |
| Cell Population | Pan-T Cell |
| Storage | Store at 2℃ - 8℃ |
Application
SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads (Column-based) is used for Positive Pan-T Cell Selection, with the Key Features, such as Fast isolation; high purity; ideal for automated workflows
Protocol
- Preparation: Prepare a single-cell suspension from Human PBMCs.
- Labeling: Add CD3 Microbeads directly to the cell suspension. Incubate to allow binding to CD3+ T Cells.
- Separation: Apply the suspension to a separation column placed in a magnetic field.
- Washing: The labeled CD3+ cells are retained in the column, while unlabeled cells (B cells, NK cells, Monocytes) are washed away.
- Elution: Remove the column from the magnetic field and elute the highly purified CD3+ T Cells.
Validation Data
Efficient Isolation of Pan-T Cells Using SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads (Column-Based)
Catalog No.GM-Tg-hg-T87075-iso-nanoIMB
To assess the purity of isolated T cells, CD3+ T cells were magnetically sorted from human peripheral blood mononuclear cells (PBMCs). Cells before and after separation were stained with APC-conjugated CD3 antibody and propidium iodide (PI), followed by flow cytometric analysis. The results demonstrated a significant increase in CD3+ T cell purity from 52.6% pre-isolated to 95.0% post-isolated, confirming successful T cell isolation.
Figure 7. Efficient Isolation of Pan-T Cells Using SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads (Column-Based). The T cells were labeled with SOLIDEX®-ISOEx Nanobeads conjugated with anti-CD3 antibody. And then these cells were enriched by using cell isolation column. The T cells in pre-isolated human PBMCs. T cells with high purity and motility were enriched.
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Background
Why Choose SOLIDEX®-ISOEx Human CD3 Isolation Nanobeads?
- Superior Recovery: Positive selection strategy ensures the capture of the maximum number of target CD3+ T cells, ideal for rare samples.
- High Specificity: The column-based washing step efficiently removes background cells, delivering exceptional purity.
- Biodegradable Nanobeads: The tiny magnetic beads are non-toxic and biodegradable, minimizing interference with cellular physiology.
- Fast Protocol: Simple incubation and separation steps allow for isolation in approximately 45 minutes.
FAQ
What are the sample requirements?
This kit is optimized for use with fresh or cryopreserved Human PBMCs. It is also compatible with Leukapheresis products (Leukopaks). Ensure high cell viability for optimal recovery.
Do the magnetic beads affect cell activation?
No. The SOLIDEX® nanobeads are extremely small and biodegradable. They are designed to minimize cross-linking and avoid inducing artificial activation of the T cells, making them safe for downstream culture and analysis.
How does the Column-based separation work?
The column contains a ferromagnetic matrix. When placed in a magnetic field, it amplifies the magnetic force, trapping the magnetically labeled CD3+ cells. This allows for vigorous washing to remove unlabeled contaminants before the target cells are eluted.
Are the separation columns reusable?
No. The columns are strictly single-use to ensure sterility and prevent cross-contamination between patient samples or experimental conditions.
What is the advantage of Column-based over Column-free methods?
Column-based positive selection typically offers higher purity. The physical matrix allows for thorough washing of the retained cells, removing non-specifically trapped contaminants that often remain in column-free "clump" based methods.
Is this protocol compatible with automated systems?
Yes, the SOLIDEX®-ISOEx column format is compatible with most standard automated magnetic separation instruments designed for column-based protocols.
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